描述

The MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. The MEGAclear™ Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Advantages of the MEGAclear™ Kit:

• Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components
• High recovery from 1 ng to 500 µg of input
• Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections
• Quick protocol—purify RNA in less than 30 minutes

Using the MEGAclear™ Kit
The protocol is quick and easy. The transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is now ready for any application requiring highly pure synthetic or in vitrotranscribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.

For Research Use Only. Not for use in diagnostic procedures.

規格

Sample Type (General):RNA
High Throughput Compatibility:Not High Throughput-Compatible (Manual)
Downstream Application:Northern Blotting, Nuclease Protection Assays
Format:Tube(s)
Product Size:20 preps
Final Product:In Vitro Transcribed RNA (Purified)
Yield (up to):500 µg
Purification Time:30 min
Number of Reactions:20 Preps
Isolation Technology:Silica Spin Column
Sample Type (Specific):Total RNA, In Vitro Transcribed RNA
For Use With (Equipment):Centrifuge, Vacuum Manifold
Starting Material (Amount):1 ng to 500 µg of dsRNA>200b or ssRA>100bp
Shipping Condition:Room Temperature

内容及儲存

Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature.