The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. The PureLink® RNA Mini Kit provides:
• Rapid, non-hazardous RNA purification capabilities
• Ready-to-use RNA for many downstream applications
Rapid and convenient column purification
The PureLink® RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1,000 µg of purified RNA from a single extraction (see table). High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.
Simplified, nontoxic RNA purification
The PureLink® RNA Mini Kit combines nontoxic guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can typically be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl, or alcohol precipitation.
Sample Type (General): | Bacteria, Blood, Tissue, Plant Samples, Cells, Yeast |
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High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
Downstream Application: | Reverse Transcriptase PCR (RT-PCR), Real-Time Quantitative PCR (qPCR), Microarray Analysis, Next-Generation Sequencing, Nuclease Protection Assays, cDNA Library Construction, Northern Blotting |
Scale: | Mini |
System: | PureLink™ |
Quantity: | 10 columns |
Product Size: | 10 preps |
Final Product: | Total RNA |
Yield (up to): | 1000 µg |
Number of Reactions: | 10 Preps |
Elution Volume - max: | 300 µl |
Elution Volume - min: | 30 µl |
Isolation Technology: | Silica Spin Column |
Starting Material (Amount): | Up to 200 mg tissue, 5 x 10^6 to 1 x 10^8 cells |
Shipping Condition: | Room Temperature |
The PureLink® RNA Mini Kit comes with enough RNA lysis solution, spin cartridges, wash tubes, recovery tubes, wash buffers, and RNase-free water to perform 10 RNA purifications. All components should be stored at room temperature. Guaranteed stable for 1 year when properly stored.