Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of Gibco® DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
With | Without |
• GlutaMAX™ | • HEPES |
• Phenol Red |
The complete formulation is available.
Gibco® DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components. Gibco® DMEM / F-12 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® DMEM / F-12 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® DMEM / F-12 product made in our Scotland facility (31331-028). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Glutamine: | GlutaMAX™-I |
---|---|
Phenol Red Indicator: | Phenol Red |
Form: | Liquid |
HEPES Buffer: | No HEPES |
Sodium Pyruvate Additive: | Sodium Pyruvate |
Serum Supplementation: | Standard Serum Supplementation |
Glucose: | High Glucose |
Product Size: | 500 mL |
Volume (Metric): | 500 ml |
Shipping Condition: | Room Temperature |
10565 - DMEM/F-12, GlutaMAX(TM)