産品介紹：
利用我們的創新和專有的脂質體共轭合成(chéng)技術，LipoJet™轉染試劑盒，運用了新型的含氟陽離子脂質體配方，同市場上的其他脂質體轉染試劑有著(zhe)顯著 的區别，其特點是轉染效率高、毒性小。LipoJet™轉染試劑盒是最強大的基因 轉導工具，适于幾乎有所有體外基因轉導的應用，包括質粒DNA、siRNA和shRNA轉染和DNA/siRNA共轉等。
試劑盒内容：
- LipoJet™ 試劑，1.0ml，足夠用1000次DNA的轉染(24孔闆中0.5 ug DNA/孔)足夠用1000次siRNA轉染(24孔闆中10 nM siRNA/孔)。
- LipoJet™轉染緩沖液(5X )，按照最大化的轉染效率配制，8.0ml濃縮液就(jiù)可以制成(chéng)40ml的工作液體。
産品特點：
- 适用于廣泛的哺乳動物細胞
- 極高效率地轉染DNA，siRNA和DNA/siRNA共轉染
- 10 nM siRNA能(néng)達到95%的沉默效應
- 無血清和抗生素幹擾
- 簡單易用的操作步驟
- 适用于高通量的應用
- 細胞毒性最低
儲存條件 ：
4⁰C儲存。若儲藏合适，産品的穩定性能(néng)保持12個月以上。
LipoJet™體外轉染試劑具有極廣譜的轉染活性。

以下圖例表明LipoJet™體外轉染試劑盒出色的DNA和siRNA轉染效率 。

A efficiency comparison of LipoJet™ reagent vs. brand name products to transfect Hela, Cos-7, NIH-3T3, CHO and HEK293 (left panel) and Cos-7 (right panel).  pEGFP-N3 cDNA (0.5 µg/well of 24-well plate) was transfected into different mammalian cells per the standard transfection protocols in presence of serum (10% FBS) and antibiotics as recommended by manufacturers.  The transfection efficiency were analyzed via FACS 48 hours post transfection.

A toxicity comparison of LipoJet™ reagent vs. brand name products to transfect Hela cells.  pEGFP-N3 cDNA (1.0 µg/well of 24-well plate) was transfected to Hela cells per the standard transfection protocols in presence of serum (10% FBS) and antibiotics as recommended by manufacturers.  The MTT assay (right panel) and phase contrast imaging (left panel) were used to analyze the cell viability 48 hours post transfection. 

A comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) and PolyJet™ transfection reagent on HEK293T cell. pEGFP-N3 cDNA (0.125 µg/well, 0.25 µg/well and 0.5 µg/per well of 24-well plate) was transfected into 293T cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™/DNA (µl/µg) ratio=2), L2K (middle panel at L2K/DNA (µl/µg) ratio=3) and PolyJet™ (lower panel at at PolyJet™/DNA (µl/µg) ratio=3) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

A comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) and PolyJet™ transfection reagent on Hela cell. pEGFP-N3 cDNA (0.125 µg/well, 0.25 µg/well and 0.5 µg/per well of 24-well plate) was transfected into Hela cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™/DNA (µl/µg) ratio=2), L2K (middle panel at L2K/DNA (µl/µg) ratio=3) and PolyJet™ (lower panel at at PolyJet™/DNA (µl/µg) ratio=3) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.



LipoJet™試劑介導的DNA/siRNA共轉染表現出色的基因沉默效應。分别由LipoJet™試劑轉染GFP的cDNA (左圖, 0.25µg/孔，24孔闆)作爲對(duì)照和共轉染GFP的cDNA (0.25µg/孔，24孔闆)/GFP的siRNA (終濃度10 nM，右圖)至HEK293細胞 (上圖), Hela細胞(中圖)和SaoS-2細胞(下圖)。轉染24小時(shí)後(hòu)，使用尼康Eclipse熒光顯微鏡檢查GFP熒光。結果顯示，GFP的cDNA和GFP的siRNA共轉染導緻GFP的表達被(bèi)顯著抑制。


Exceptional DNA/siRNA co-transfection efficiency on HUVEC. Co-transfection of mCherry cDNA (0.10 µg per well of 24-well plate) and FITC conjugated siRNA (final 30 nM per well of 24-well plate) to HUVEC with LipoJet™ reagent gave rise to 60% mCherry+ (phase contrast overlapped with mCherry imaging, left panel) and nearly 100% FITC-siRNA+ (phase contrast overlapped with FITC imaging, right panel) HUVEC 24 hours after transfection.  The pictures were given from Dr. Pan Kong of USC as courtesy.  


Excellent silencing of endogenously expressed KIF11 (also known as EG5) in HEK293 (upper panel) and Hela (lower panel) cells with LipoJet™ reagent at 10 nM EG5 siRNA. KIF11 (also known as EG5) encodes a motor protein that belongs to the kinesin-like protein family involved in chromosome positioning and bipolar spindle formation during cell mitosis. A reduction in KIF11 levels causes mitotic arrest. LipoJet™ reagent effectively delivers EG5 siRNA (final 10 nM) to HEK293 and Hela cells, leading to more than 80% of "round-up" phenotype of HEK293 and Hela cells 24h post transfection over negative control (final 10 nM with sham EG5 siRNA). The phenotype of "rounded-up" HEK293 and Hela cells were visualized 24h post transfection with a Nikon microscope.