• Assay Type ： Solid Phase Sandwich ELISA

• Format：  96-well strip plate

• Assay Length ：4.5 hours

• Sample Type & Volume Required Per Well Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

• Sensitivity：14.3 pg/mL

• Assay Range：31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

• Specificity：Natural and recombinant mouse and rat IL-33

• Cross-reactivity：< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

• Interference：No significant interference observed with available related molecules.

The Quantikine Mouse/Rat IL-33 immunoassay is a 4.5 hour solid-phase ELISA designed to measure IL-33 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse mature IL-33 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate both recombinant mouse and rat IL-33. Results obtained using natural IL-33 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-33.