Description:
PepMute™ Plus siRNA & DNA Transfection Reagent is an upgraded version of PepMute™ reagent (Cat # SL100566) by crosslinking pre-screened hydrophobic amino acids to its backbone. With our proprietary pH Dependent Conformational Change (PDCC) technology, the transfection reagent which contains 36 amino acids long's peptide backbone was formulated by addition of pre-screened hydrophobic amino acids to all amine groups, conferringPepMute™ Plus reagent an ability to self-assembly during formation of transfection complex and making PepMute™ reagent a versatile and most powerful gene delivery tool which provides more than 95% silencing efficiency at 1 nM siRNA in variety of mammalian cells. PepMute™ Plus Reagent have been validated to effectively and reproducibly transfect single DNA or siRNA or co-transfect DNA/siRNA to variety of mammalian cells.

Size & content:
- PepMute™ Plus Transfection Reagent, 1.0 mL, sufficient for ~1000 reactions based on transfecting 2.5 pmols siRNA in 24-well plate
- PepMute™ Plus Transfection Buffer(5x ), formulated for maximal transfection efficiency, 8.0 mL

Storage:
Store at 4 °C for PepMute™ Plus Reagent and RT for PepMute™ Transfection Buffer (5x ).  If stored properly, the product is stable for 12 months or longer.

Advantages: 
- Effective knockdown (95%) with only 0.5 pmol siRNA (1.0 nM siRNA in 24-well plate)
- Formulated for hard-to-transfect mammalian cells
- One tube reaction and easy transfection protocol
- Best for broad spectrum especially hard-to-transfect mammalian cells
- Very low cytotoxicity