One-step RT-qPCR Master Mix for sensitive and reliable multiplex detection of viral RNA targets and internal controls.
For general laboratory use.
Overview:
The combination of a unique reverse transcriptase enzyme solution and the aptamer-mediated hot start AptaTaq Fast DNA Polymerase, guarantees fast and highly specific one-step RT-qPCR of viral RNA targets isolated from a variety of human sample materials.
Unique recombinant reverse transcriptase for efficient and robust reverse transcription of RNA isolated from a wide range of materials, including difficult sample materials (e.g., blood, plasma, serum, and stool).
Highly sensitive reverse transcription and multiplex qPCR detection.
Fast results and specific priming due to an aptamer-mediated hot start protocol.
Especially suited for automated workflows due to high thermostability of reverse transcriptase and AptaTaq Fast DNA Polymerase.
Contents:
RT-Enzyme Solution, 200x conc.
RT-PCR Reaction Mix, 5x conc.
Water, PCR grade
Application:
The LightCycler® Multiplex RNA Virus Master consists of a unique recombinant reverse transcriptase (200x concentrated) and a complete 5x PCR reaction mix with AptaTaq Fast DNA Polymerase, dNTPs, and a special reaction mix for multiplex qPCR. AptaTaq Fast DNA Polymerase is a thermostable, recombinant Taq polymerase with aptamer-mediated, reversible hot start. It is immediately activated by heating, which allows specific priming and fast PCR.