Alexa Fluor® 647 Mouse Anti-Human CD107A  Clone  H4A3  (RUO)

• Brand

• BD Pharmingen™

• Alternative Name

• LAMP1; LAMP-1; LAMPA; LGP120

• Vol. Per Test

• 5 µl

• Isotype

• Mouse BALB/c IgG1, κ

• Reactivity

• Human (QC Testing) 

• Application

• Intracellular staining (flow cytometry) (Routinely Tested) 
  

• Immunogen

• Human Adult Adherent Peripheral Blood Cells

• Workshop No.

• V P008

• Storage Buffer

• Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

DESCRIPTION

The H4A3 monoclonal antibody specifically binds to the heavily glycosylated 110 kDa Lysosomal-associated membrane protein 1, LAMP-1. LAMP-1 is a widely expressed intracellular antigen. It is also expressed on the surface of activated platelets, PHA-activated lymphocytes, cytotoxic T cells and NK cells, and some tumor cell lines, including U937 and KG1a. LAMP-1 has been shown to be a ligand for E-selectin-mediated cell adhesion. LAMP-1 and LAMP-2 (CD107b) are carriers for poly-N-acetyllactosamines and are able to display sialyl Le[x] termini.

FORMAT

• Format

• Alexa Fluor® 647

• Excitation Source

• Red 633 nm

• Excitation Max

• 650 nm

• Emission Max

• 668 nm

Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.