Alexa Fluor® 647 Mouse Anti-Human CD107A Clone H4A3 (RUO)
Brand
BD Pharmingen™
Alternative Name
LAMP1; LAMP-1; LAMPA; LGP120
Vol. Per Test
5 µl
Isotype
Mouse BALB/c IgG1, κ
Reactivity
Human (QC Testing)
Application
Intracellular staining (flow cytometry) (Routinely Tested)
Immunogen
Human Adult Adherent Peripheral Blood Cells
Workshop No.
V P008
Storage Buffer
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
The H4A3 monoclonal antibody specifically binds to the heavily glycosylated 110 kDa Lysosomal-associated membrane protein 1, LAMP-1. LAMP-1 is a widely expressed intracellular antigen. It is also expressed on the surface of activated platelets, PHA-activated lymphocytes, cytotoxic T cells and NK cells, and some tumor cell lines, including U937 and KG1a. LAMP-1 has been shown to be a ligand for E-selectin-mediated cell adhesion. LAMP-1 and LAMP-2 (CD107b) are carriers for poly-N-acetyllactosamines and are able to display sialyl Le[x] termini.
FORMAT
Format
Alexa Fluor® 647
Excitation Source
Red 633 nm
Excitation Max
650 nm
Emission Max
668 nm
Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.