ZYMUPHEN MP-Activity

(# 521096)

Functional assay for the measurement of microparticles’ procoagulant activity in plasma

FOR RESEARCH USE ONLY.

NOT FOR USE IN DIAGNOSTIC PROCEDURES

INTENDED USE:

The ZYMUPHEN MP-Activity kit is a functional assay proposed for the measurement of microparticles’ procoagulant activity in human plasma, in vitro, using automated or manual method.

This kit is for research use only and should not be used for patient diagnosis or treatment. ASSAY PRINCIPLE:

The diluted assayed plasma sample, supplemented with calcium, Factor Xa and thrombin inhibitors, is introduced into one of the microplate wells coated with Streptavidine and biotinylated Annexin V, then incubated. Following a washing step, the Factor Xa-Va mixture containing calcium, then the purified prothrombin, are introduced. When present in the tested sample, microparticles bind to Annexin V and expose their phospholipids surface, thus allowing to FXa-FVa, in presence of calcium, to activate prothrombin into thrombin. The phospholipids concentration is then the limiting factor. There is a direct relationship between the phospholipids concentration and the amount of thrombin generation, which is measured via its specific activity on the thrombin substrate. The reaction is stopped with 2% Citric Acid and Absorbance is measured at 405 nm (A405). TESTED SAMPLES:

Citrated human plasma, prepared from citrated blood collected using conditions, which avoid any activation (refer paragraph “sample collection and preparation”).