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Protein A Sepharose CL-4B, 1.5 g

Protein A Sepharose CL-4B, 1.5 g

Protein A Sepharose CL-4B is a classic, well documented medium for immunoglobulin purification and fractionation, and is suitable for immunoprecipitation procedures.

• Well documented for immunoglobulin purification and fractionation

• Strong binding to Fc region of IgG

• High capacity - 20 mg human IgG/ml.

• Suited for immunoprecipitation procedures

Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.