Use ProteoMiner Protein Enrichment Small-Capacity Kits to simultaneously concentrate low-abundance proteins and lower the concentration of high-abundance proteins in ≥10 mg of sample. The protein complexity of the sample is maintained.
The enrichment protocol uses a large, highly diverse library of combinatorial peptide ligands bound to beads. After high-abundance proteins have saturated all their high-affinity ligands, the unbound excess protein is washed away. Most medium- and low-abundance proteins will not saturate their ligands, resulting in no excess to be washed away.
Knowledge of the identity and characteristics of the proteins within a sample is not required
Compatible with a wide range of sample types
Narrows the range of protein concentrations within a sample
Can be used for differential expression analysis
Compatible with most downstream applications including 1-D and 2-D electrophoresis and mass spectrometry
For fractionation of the protein sample after binding to the beads, the ProteoMiner Protein Enrichment Small-Capacity Kit can be used with the ProteoMiner Sequential Elution Reagents (1633003). The elution kit contains four different elution buffers, providing multiple options for successive elution of populations of proteins with different characteristics. The ProteoMiner Protein Enrichment Small-Capacity Kit and Sequential Elution Reagents can be purchased together in one kit, the ProteoMiner Sequential Elution Small-Capacity Kit (1633010). Sequential elution has been optimized to prepare samples for SELDI ProteinChip analysis. Elution reagents are not compatible with direct use of fractions in 2-D gel electrophoresis.
Kit Contents
Spin columns, 10; 500 µl bead slurry (4% beads, 20% v/v EtOH), 20 µl settled bead volume
Wash Buffer, 150 mM NaCl, 10 mM NaH2PO4, pH 7.4, 50 ml
Elution Reagent, lyophilized 8 M urea, 2% CHAPS, 2 vials
Rehydration Reagent, 5% acetic acid, 5 ml
Capless 2 ml centrifuge tubes, 20
Capped 2 ml centrifuge tubes, 10
Instruction Manual
Quick Start Guide
| ProteoMiner Protein Enrichment and Elution Kits | Capacity | Number of Samples | Catalog Number |
| Small-Capacity Enrichment Kit | ≥10 mg | 10 | 1633006 |
| Introductory Small-Capacity Enrichment Kit | ≥10 mg | 2 | 1633008 |
| Large-Capacity Enrichment Kit | ≥50 mg | 10 | 1633007 |
| Introductory Large-Capacity Enrichment Kit | ≥50 mg | 2 | 1633009 |
| Sequential Elution Reagents |
| 10 | 1633003 |
| Small-Capacity Elution Kit (combines 1633006 and 2633003) | ≥10 mg | 10 | 1633010 |
| Large-Capacity Elution Kit (combines 1633007 and 2633003) | ≥50 mg | 10 | 1633011 |
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