Biotin-labeled synthetic analog of dsRNA

Poly(I:C) HMW Biotin was chemically labeled by covalent coupling of biotin with poly(I:C) HMW (High molecular weight).

Polyinosinic-polycytidylic acid (poly(I:C)) is a synthetic analog of double stranded RNA (dsRNA), a molecular pattern associated with viral infection.

Both natural and synthetic dsRNAs are known to induce type I interferons (IFN) and other cytokines production.

Poly(I:C) is recognized by Toll-like receptor 3 (TLR3) [1, 2].

1. Alexopoulou L. et al., 2001. Recognition of double-stranded RNA and activation of NF-kB by Toll-like receptor 3. Nature, 413:732-8.
2. Matsumoto M. et al., 2002. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. BBRC 293:1364-9.

Specifications

Specificity: TLR3 agonist

Working Concentration: 30 ng - 10 μg/ml

Contents

Poly(I:C) HMW Biotin is provided lyophilized:

• 10 µg Poly(I:C) HMW Biotin

• 1.5 ml endotoxin-free water

Poly(I:C) HMW Biotin is shipped at room temperature

Upon receipt it should be stored at -20°C for up to 6 months.

Upon resuspension, Poly(I:C) HMW Biotin should be aliquoted and stored at -20˚C.

Resuspended product is stable 3 months at -20°C.

Avoid repeated freeze-thaw cycles.

Citations

TDS
MSDS