Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using HT‑2 mouse T cells. The ED50 for this effect is 0.3‑1.5 ng/mL.
Source
E. coli-derived mouse IL-4 protein
His23-Ser140, with an N-terminal Met
Accession #
P07750
N-terminal Sequence
Analysis
His23
Predicted Molecular Mass
14 kDa