Anti-Rat and Anti-Hamster Ig κ /Negative Control Compensation Particles Set 

• Brand

• BD™ CompBead

• Application

• Flow cytometry (Routinely Tested) 

  
  

• DESCRIPTION

• The BD™ CompBeads Compensation Particles Anti-Rat/Hamster Ig, κ Set are polystyrene microparticles which are used to optimize fluorescence compensation settings for multicolor flow cytometric analyses. The set provides two populations of microparticles, the BD CompBeads Anti-Rat/Hamster Ig, κ particles, which bind any rat or hamster κ light chain-bearing immunoglobulin, and the BD CompBeads Negative Control (FBS) Particles, which has no binding capacity. When mixed together with a fluorochrome-conjugated rat or hamster antibody, the BD CompBeads provide distinct positive and negative (background fluorescence) stained populations which can be used to set compensation levels manually or using instrument set-up software. Since the compensation adjustments are made using the same fluorochrome-labeled antibody to be used in the experiment, this method allows the investigator to more accurately establish compensation corrections for spectral overlap for any combination of fluorochrome-labeled antibodies (without having to use valuable tissue samples or hard-dyed beads with potentially mismatched fluorescence spectra). Use of the BD CompBeads is highly recommended for use in all experiments using tandem dye (i.e., PE-Cy7, APC-Cy7, etc.) conjugates, which may have distinct spectral characteristics for each conjugate.