BD PE-Cy™7 Mouse Anti-Human TNF Clone MAb11 (RUO) 560678
PE-Cy™7 Mouse Anti-Human TNF
Clone MAb11 (RUO)
BrandBD Pharmingen™
Alternative NameTumor necrosis factor alpha; TNF-a; TNF-α; TNFSF2; Cachectin
Vol. Per Test5 µl
IsotypeMouse IgG1, κ
ReactivityRhesus, Baboon, Cynomolgus (QC Testing) Human (Tested in Development)
ApplicationIntracellular staining (flow cytometry) (Routinely Tested)
ImmunogenRecombinant Human TNF
Storage BufferAqueous buffered solution containing BSA and ≤0.09% sodium azide.
FORMAT
FormatPE-Cy™7
Excitation SourceBlue 488 nm,Green 532 nm,Yellow/Green 561 nm
Excitation Max496 nm
Emission Max785 nm
PE-Cy™7 is a tandem fluorochrome that combines PE and a cyanine dye. PE-Cy7 conjugated reagents are as bright as PE conjugates. PE-Cy7 is particularly sensitive to photo-induced degradation, resulting in loss of fluorescence and changes in fluorescence spillover. Extreme caution must be taken to avoid light exposure and prolonged exposure to paraformaldehyde fixative. Fixed cells should be analyzed within 4 hours of fixation in paraformaldehyde or transferred to a paraformaldehyde-free buffer for overnight storage.