APC-H7 Mouse Anti-Human CD4 

Clone  L200   (RUO)

BrandBD Pharmingen™

Alternative NameL3T4 ; T-cell surface antigen T4/Leu-3; W3/25 ; CD4 antigen (p55)

Vol. Per Test5 µl

IsotypeMouse BALB/c IgG1, κ

ReactivityRhesus, Cynomolgus, Baboon (QC Testing)  Human (Tested in Development) 

ApplicationFlow cytometry (Routinely Tested) 

ImmunogenHuman HPB-ALL Cell Line

Storage BufferAqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.

Description

The L200 monoclonal antibody specifically binds to the human form of the 56 kDa transmembrane glycoprotein, CD4, which is present on the T-helper/inducer subset of normal human donor peripheral blood lymphocytes. The L200 antibody also cross-reacts with a subset of CD3-positive peripheral blood lymphocytes, but not monocytes, of both Rhesus and Cynomolgus Macaque monkeys. Cross-reactivity on both lymphocytes and monocytes (weak) from baboons is also observed. CD4 distribution on lymphocytes is similar for both human and monkey cells, with the majority of CD4-positive lymphocytes being CD8-negative and lacking reactivity with antibodies to B- or NK-cell markers.

FORMAT

• FormatAPC-H7

• Excitation SourceRed 633 nm

• Excitation Max640 nm

• Emission Max785 nm

APC-H7 is an APC-cyanine tandem fluorochrome, which uses an analog of Cy7 and has similar spectral properties to APC-Cy7. APC-H7 conjugates provide greater stability in light and paraformaldehyde fixatives and have less spillover into the APC channel than APC-Cy7 conjugates. APC-H7 conjugates are typically 75% as bright as equivalent APC-Cy7 conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, APC-Cy7 and APC-H7 cannot be used simultaneously.