Zeocin™ | Selection antibiotic: cell culture tested, sterile reagent

InvivoGen is the sole worldwide producer of Zeocin™.

Zeocin™ is a formulation of phleomycin D1, a copper-chelated glycopeptide antibiotic produced by Streptomyces CL990. Zeocin™ causes cell death by intercalating into DNA and cleaving it.

The action of Zeocin™ is effective on most aerobic cells. Therefore, Zeocin™ is a popular and effective antibiotic for the selection of vectors bearing the Sh ble gene in a variety of cells types (bacteria, eukaryotic microorganisms, plant and animal cells).

Resistance to Zeocin™ is conferred by the Sh ble gene product from Streptoalloteichus hindustanus, which inactivates Zeocin™ upon binding to the antibiotic [1-3].

Sh ble is a small 370bp-sized gene and is carried in a number of InvivoGen’s vectors.

Typically, mammalian cells are sensitive to Zeocin™ concentrations of 50-400 µg/ml, and bacteria to 25 µg/ml.

pecifications

Product concentration: 100 mg/ml in solution

CAS number: 11006-33-0

Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.

Endotoxin level: < 1 EU/mg

Physicochemical characterization: HPLC, pH, appearance

Cell-culture tested: potency validated in Zeocin™-sensitive and Zeocin™-resistant mammalian cell lines

Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in Zeocin™-resistant cells

Contents

Zeocin™ is supplied as a sterile filtered blue solution at 100 mg/ml in HEPES buffer.

This product is available in three pack sizes:

• ant-zn-1: 10 x 1 ml (1 g)

• ant-zn-5: 50 x 1 ml (5 g)

• ant-zn-5b: 1 x 50 ml (5 g)

Zeocin™ is also supplied as a blue powder:

• ant-zn-1p: 1 x 1 g

• ant-zn-5p: 1 x 5 g

Zeocin™ is shipped at room temperature.

Upon receipt it should be stored at 4°C or -20°C.

 Zeocin™ is a harmful compound. Refer to safety data sheet for handling instructions.