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Product Details
SynonymsNACHT-, LRR- and PYD-containing Protein 3; NLRP3; Cryopyrin; Cold Autoinflammatory Syndrome 1 Protein
Product TypeMonoclonal Antibody
Properties
CloneCryo-2
IsotypeMouse IgG2b
Source/HostPurified from concentrated hybridoma tissue culture supernatant.
Immunogen/AntigenRecombinant mouse NLRP3/NALP3 (pyrin domain/aa 1-93).
Application

Western Blot (see online protocol): (1μg/ml)
Immunocytochemistry: (see J. Virol. 85, 13019 (2011) and Nat. Immunol. 13, 255 (2012))
Immunoprecipitation: (in RIPA buffer, see Mol. Cell 49, 331 (2012))
Immunohistochemistry: (1:200, for a protocol see Nature 493, 67 (2012))
ChIP assay: 2µg/DNA of 10E6 cells (see Nat. Immunol. 16, 859 (2015))

CrossreactivityHuman
Mouse
Specificity

Recognizes mouse and human NLRP3/NALP3.

Purity≥95% (SDS-PAGE)
Purity DetailProtein G-affinity purified.
Concentration1mg/ml
FormulationLiquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Shipping and Handling
ShippingBLUE ICE
Short Term Storage+4°C
Long Term Storage-20°C
Handling AdviceAfter opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/StabilityStable for at least 1 year after receipt when stored at -20°C.